Publications

Scientific Publications 

  1.  Casabonne D, Gracia E, Espinosa A, Bustamante M, Benavente Y, Robles C, Costas L, Alonso E, Gonzalez-Barca E, Tardón A, Dierssen-Sotos T, Vázquez EG, Aymerich M, Campo E, Jiménez-Moleón JJ, Marcos-Gragera R, Castaño-Vinyals G, Aragones N, Pollan M, Kogevinas M, Urtiaga C, Amiano P, Moreno V, de Sanjose S. Fruit and vegetable intake and vitamin C transporter gene (SLC23A2) polymorphisms in chronic lymphocytic leukaemia. Eur J Nutr 2016 Feb 2. [Epub ahead of print].
  2. Delgado J, Bielig T, Bonet L, Carnero-Montoro E, Puente XS, Colomer D, Bosch E, Campo E, Lozano F. Impact of the functional CD5 polymorphism A471V on the response of chronic lymphocytic leukaemia to conventional chemotherapy regimens. Br J Haematol 2016. [Epub ahead of print].
  3. Martínez-Trillos A, Navarro A, Aymerich M, Delgado J, López-Guillermo A, Campo E, Villamor N. Clinical impact of MYD88 mutations in chronic lymphocytic leukemia. Blood 2016;127(12):1611-3.
  4. Nadeu F, Delgado J, Royo C, Baumann T, Stankovic T, Pinyol M, Jares P, Navarro A, Martín-García D, Beà S, Salaverria I, Oldreive C, Aymerich M, Suárez-Cisneros H, Rozman M, Villamor N, Colomer D, López-Guillermo A, González M, Alcoceba M, Terol MJ, Colado E, Puente XS, López-Otín C, Enjuanes A, Campo E. Clinical impact of clonal and subclonal TP53, SF3B1, BIRC3, NOTCH1 and ATM mutations in chronic lymphocytic leukemia. Blood 2016;127(17):2122-30.
  5. Montraveta A, Lee-Vergés E, Roldán J, Jiménez L, Cabezas S, Clot G, Pinyol M, Xargay-Torrent S, Rosich L, Arimany-Nardí C, Aymerich M, Villamor N, López-Guillermo A, Pérez-Galán P, Roué G, Pastor-Anglada M, Campo E, López-Guerra M, Colomer D. CD69 expression potentially predicts response to bendamustine and its modulation by ibrutinib or idelalisib enhances cytotoxic effect in chronic lymphocytic leukemia. Oncotarget 2016;7(5):5507-20. 
  6. Salaverria I, Martín-Garcia D, López C, Clot G, García-Aragonés M, Navarro A, Delgado J, Baumann T, Pinyol M, Martin-Guerrero I, Carrió A, Costa D, Queirós AC, Jayne S, Aymerich M, Villamor N, Colomer D, González M, López-Guillermo A, Campo E, Dyer MJ, Siebert R, Armengol L, Beà S. Detection of chromothripsis-like patterns with a custom array platform for chronic lymphocytic leukemia. Genes Chromosomes Cancer 2015;54(11):668-80.
  7. Casabonne D, Benavente Y, Robles C, Costas L, Alonso E, Gonzalez-Barca E,Tardón A, Dierssen-Sotos T, Vázquez EG, Aymerich M, Campo E, Castaño-Vinyals G,Aragones N, Pollan M, Kogevinas M, Juwana H, Middeldorp J, de Sanjose S. Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in aSpanish multicentric case-control study. Infect Agents Cancer 2015; 10:5.
  8. Rodríguez D, Bretones G, Arango JR, Valdespino V, Campo E, Quesada V, López-Otín C.  Molecular pathogenesis of CLL and its evolution. Int J Hematol 2015; 101(3):219-28.
  9. Puente XS , Beà S,  Valdés-Mas R, Villamor N, Gutiérrez-Abril J, Martín-Subero JI, Munar M, Rubio-Pérez C,  Jares P, Aymerich M, Baumann T, Beekman R, Belver L, Carrió A, Castellano G, Clot G, Colado E, Colomer D,  Costa D, Delgado J, Enjuanes A, Estivill X, Ferrando AA, Gelpí JL, González B, González S, González M, Gut M,  Hernández-Rivas JM, López-Guerra M, Martín-García D, Navarro A, Nicolás P,  Orozco M,  Payer AR, Pinyol M,  Pisano DG, Puente DA, Queirós AC, Quesada V, Romeo-Casabona CM, Royo C, Royo R, Rozman M,  Russiñol N, Salaverría I, Stamatopoulos K, Stunnenberg HG, Tamborero D, Terol MJ, Valencia A, López-Bigas N,  Torrents D, Gut I, López-Guillermo A, López-Otín C, Campo E. Non-Coding Recurrent Mutations in Chronic Lymphocytic LeukaemiaNature 2015; published online.
  10. Rodríguez D, Bretones G, Quesada V, Villamor N, Arango JR, López-Guillermo A, Ramsay AJ, Baumann T, Quirós PM, Navarro A, Royo C, Martín-Subero JI, Campo E, López-Otín C. Mutations in CHD2 cause defective association with active chromatin in chronic lymphocytic leukemiaBlood 2015;126(2):195-202. 
  11. Ecker S, Pancaldi V, Rico D, Valencia A. Higher gene expression variability in the more aggressive subtype of chronic lymphocytic leukemiaGenome Medicine 2015;7:8.
  12. López-Guerra M, Xargay-Torrent S, Rosich L, Montraveta A, Roldán J, Matas-Céspedes A, Villamor N, Aymerich M, López-Otín C, Pérez-Galán P, Roué G, Campo E, Colomer D. The γ-secretase inhibitor PF-03084014 combined with fludarabine antagonizes migration, invasion and angiogenesis in NOTCH1-mutated CLL cells. Leukemia 2015;29(1):96-106.
  13. Moncunill V, Gonzalez S, Beà S, Andrieux LO, Salaverria I, Royo C, Martinez L, Puiggròs M, Segura-Wang M, Stütz AM, Navarro A, Royo R, Gelpí JL, Gut IG, López-Otín C, Orozco M, Korbel JO, Campo E, Puente XS, Torrents D. Comprehensive characterization of complex structural variations in cancer by directly comparing genome sequence reads. Nat Biotechnol 2014;32(11):1106-12.
  14. Delgado J, Salaverria I, Baumann T, Martínez-Trillos A, Lee E, Jiménez L, Navarro A, Royo C, Santacruz R, López C, Payer AR, Colado E, González M, Armengol L, Colomer D, Pinyol M, Villamor N, Aymerich M, Carrió A, Costa D, Clot G, Giné E, López-Guillermo A, Campo E, Beà S. Genomic complexity and IGHV mutational status are key predictors of outcome of chronic lymphocytic leukemia patients with TP53 disruption. Haematologica 2014;99(11):e231-4. 
  15. Martínez-Trillos A, Pinyol M, Navarro A, Aymerich M, Jares P, Juan M, Rozman M, Colomer D, Delgado J, Giné E, González-Díaz M, Hernández-Rivas JM, Colado E, Rayón C, Payer AR, Terol MJ, Navarro B, Quesada V, Puente XS, Rozman C, López-Otín C, Campo E, López-Guillermo A, Villamor N. Mutations in TLR/MYD88 pathway identify a subset of young chronic lymphocytic leukemia patients with favorable outcome. Blood 2014;123(24):3790-6. 
  16. Santacruz R, Villamor N, Aymerich M, Martínez-Trillos A, López C, Navarro A, Rozman M, Beà S, Royo C, Cazorla M, Colomer D, Giné E, Pinyol M, Puente XS, López-Otín C, Campo E, López-Guillermo A, Delgado J. The prognostic impact of minimal residual disease in patients with chronic lymphocytic leukemia requiring first-line therapy. Haematologica 2014;99(5):873-80. 
  17. Ferreira PG, Jares P, Rico D, Gómez-López G, Martínez-Trillos A, Villamor N, Ecker S, González-Pérez A, Knowles DG, Monlong J, Johnson R, Quesada V, Djebali S, Papasaikas P, López-Guerra M, Colomer D, Royo C, Cazorla M, Pinyol M, Clot G, Aymerich M, Rozman M, Kulis M, Tamborero D, Gouin A, Blanc J, Gut M, Gut I, Puente XS, Pisano DG, Martin-Subero JI, López-Bigas N, López-Guillermo A, Valencia A, López-Otín C, Campo E, Guigó R. Transcriptome characterization by RNA sequencing identifies a major molecular and clinical subdivision in CLLGenome Research 2014;24: 212-26.
  18. Beà S, Valdés-Mas R, Navarro A, Salaverria I, Martín-Garcia D, Jares P, Giné E, Pinyol M, Royo C, Nadeu F, Conde L, Juan M, Clot G, Vizán P, Di Croce L, Puente DA, López-Guerra M, Moros A, Roue G, Aymerich M, Villamor N, Colomo L, Martínez A, Valera A, Martín-Subero JI, Amador V, Hernández L, Rozman M, Enjuanes A, Forcada P, Muntañola A, Hartmann EM, Calasanz MJ, Rosenwald A, Ott G, Hernández-Rivas JM, Klapper W, Siebert R, Wiestner A, Wilson WH, Colomer D, López-Guillermo A, López-Otín C, Puente XS, Campo E. Landscape of somatic mutations and clonal evolution in mantle cell lymphoma. Proc Natl Acad Sci U S A 2013;110(45):18250-5.
  19. López C, Delgado J, Costa D, Villamor N, Navarro A, Cazorla M, Gómez C, Arias A, Muñoz C, Cabezas S, Baumann T, Rozman M, Aymerich M, Colomer D, Pereira A, Cobo F, López-Guillermo A, Campo E, Carrió A. Clonal evolution in chronic lymphocytic leukemia: Analysis of correlations with IGHV mutational status, NOTCH1 mutations and clinical significanceGenes Chromosomes Cancer 2013;5210:920-7.
  20. Nicolás P. "Las bases nitrogenadas en bases de datos. El régimen jurídico de los archivos de información genética con fines de investigación biomédica", en Comunicaciones en Propiedad Industrial y Derecho de la Competencia, ISSN 1579-3494, Nº. 68, enero-abril, 2013, págs. 191-224. 
  21. Bassaganyas L, Beà S, Escaramís G, Tornador C, Salaverria I, Zapata L, Drechsel O, Ferreira PG, Rodriguez-Santiago B, Tubio JM, Navarro A, Martín-García D, López C, Martínez-Trillos A, López-Guillermo A, Gut M, Ossowski S, López-Otín C, Campo E, Estivill X. Sporadic and reversible chromothripsis in chronic lymphocytic leukemia revealed by longitudinal genomic analysisLeukemia 2013;27(12):2376-9.
  22. Rodríguez D, Ramsay AJ, Quesada V, Garabaya C, Campo E, Freije JM, López-Otín C. Functional analysis of sucrase isomaltase mutations from chronic lymphocytic leukemia patientsHum Mol Genet 2013;22(11):2273-82.
  23. Ramsay AJ, Quesada V, Foronda M, Conde L, Martínez-Trillos A, Villamor N, Rodríguez D, Kwarciak A, Garabaya C, Gallardo M, López-Guerra M, López-Guillermo A, Puente XS, Blasco MA, Campo E, López-Otín C. POT1 mutations cause telomere dysfunction in chronic lymphocytic leukaemiaNat Genet 2013;45(5):526-30.
  24. Villamor N, Conde L, Martínez-Trillos A, Cazorla M, Navarro A, Beà S, López C, Colomer D, Pinyol M, Aymerich M, Rozman M, Abrisqueta P, Baumann T, Delgado J, Giné E, González-Díaz M, Hernández JM, Colado E, Payer AR, Rayon C, Navarro B, José Terol M, Bosch F, Quesada V, Puente XS, López-Otín C, Jares P, Pereira A, Campo E, López-Guillermo A. NOTCH1 mutations identify a genetic subgroup of chronic lymphocytic leukemia patients with high risk of transformation and poor outcomeLeukemia 2013;27(5):1100-6. 
  25. Ramsay AJ, Rodríguez D, Villamor N, Kwarciak A, Tejedor JR, Valcárcel J, López-Guillermo A, Martínez-Trillos A, Puente XS, Campo E, López-Otín C, Quesada V. Frequent somatic mutations in components of the RNA processing machinery in chronic lymphocytic leukemia. Leukemia 2013;27(7):1600.3. 
  26. Romeo-Casabona C, Nicolás P, Knoppers BM, Joly Y, Wallace SE, Chalmers D, Dyke S, Kennedy K, Troncoso A, Kaan T, Rial-Sebbag E.  Legal Aspects of Genetic Databases for International Biomedical Research: The Example of the International Cancer Genome Consortium (ICGC)Revista de Derecho y Genoma Human / Law and the Human Genome Review 2012 Jul-Dec; (37):15-34.
  27. Valdés-Mas R, Bea S, Puente DA, López-Otín C, Puente XS. Estimation of Copy Number Alterations from Exome Sequencing Data. PLoS ONE 2012;7(12):e51422. 
  28. López C, Delgado J, Costa D, Conde L, Ghita G, Villamor N, Navarro A, Cazorla M, Gómez C, Arias A, Muñoz C, Baumann T, Rozman M, Aymerich M, Colomer D, Cobo F, Campo E, López-Guillermo A, Montserrat E, Carrió A. Different distribution of NOTCH1 mutations in chronic lymphocytic leukemia with isolated trisomy 12 or associated with other chromosomal alterationsGenes Chromosomes Cancer 2012;51(9):881-9.
  29. Kulis M, Heath S, Bibikova M, Queirós AC, Navarro A, Clot G, Martínez-Trillos A, Castellano G, Brun-Heath I, Pinyol M, Barberán-Soler S, Papasaikas P, Jares P, Beà S, Rico D, Ecker S, Rubio M, Royo R, Ho V, Klotzle B, Hernández L, Conde L, López-Guerra M, Colomer D, Villamor N, Aymerich M, Rozman M, Bayes M, Gut M, Gelpí JL, Orozco M, Fan JB, Quesada V, Puente XS, Pisano DG, Valencia A, López-Guillermo A, Gut I, López-Otín C, Campo E, Martín-Subero JI. Epigenomic analysis detects widespread gene-body DNA hypomethylation in chronic lymphocytic leukemia. Nat Genet 2012;44(11):1236-42. 
  30. Puente XS, Pinyol M, Quesada V, Conde L, Ordóñez GR, Villamor N, Escaramis G, Jares P, Beà S, González-Díaz M, Bassaganyas L, Baumann T, Juan M, López-Guerra M, Colomer D, Tubío JM, López C, Navarro A, Tornador C, Aymerich M, Rozman M, Hernández JM, Puente DA, Freije JM, Velasco G, Gutiérrez-Fernández A, Costa D, Carrió A, Guijarro S, Enjuanes A, Hernández L, Yagüe J, Nicolás P, Romeo-Casabona CM, Himmelbauer H, Castillo E, Dohm JC, de Sanjosé S, Piris MA, de Alava E, San Miguel J, Royo R, Gelpí JL, Torrents D, Orozco M, Pisano DG, Valencia A, Guigó R, Bayés M, Heath S, Gut M, Klatt P, Marshall J, Raine K, Stebbings LA, Futreal PA, Stratton MR, Campbell PJ, Gut I, López-Guillermo A, Estivill X, Montserrat E, López-Otín C, Campo E. Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia. Nature 2011;475(7354):101-5.
  31. Quesada V, Conde L, Villamor N, Ordóñez GR, Jares P, Bassaganyas L, Ramsay AJ, Beà S, Pinyol M, Martínez-Trillos A, López-Guerra M, Colomer D, Navarro A, Baumann T, Aymerich M, Rozman M, Delgado J, Giné E, Hernández JM, González-Díaz M, Puente DA, Velasco G, Freije JM, Tubío JM, Royo R, Gelpí JL, Orozco M, Pisano DG, Zamora J, Vázquez M, Valencia A, Himmelbauer H, Bayés M, Heath S, Gut M, Gut I, Estivill X, López-Guillermo A, Puente XS, Campo E, López-Otín C. Exome sequencing identifies recurrent mutations of the splicing factor SF3B1 gene in chronic lymphocytic leukemia. Nat Genet 2011;44(1):47-52.

Editorials, Revisions and Others

  1. Colomer D, Campo E. Unlocking new therapeutic targets and resistance mechanisms in mantle cell lymphoma. Cancer Cell 2014;25(1):7-9.
  2. Villamor N, López-Guillermo A, López-Otín C, Campo E. Next-generation sequencing in chronic lymphocytic leukemia. Semin Hematol 2013;50(4):286-95.
  3. Kulis M, Queirós AC, Beekman R, Martín-Subero JI. Intragenic DNA methylation in transcriptional regulation, normal differentiation and cancer. Biochim Biophys Acta 2013;1829(11):1161-74. 
  4. Delgado J, Villamor N. Chronic lymphocytic leukemia in young individuals revisited [Editorial]. Haematologica 2014;99:4-5. 
  5. Quesada V, Ramsay AJ, Rodríguez D, Puente XS, Campo E, López-Otín C. The genomic landscape of chronic lymphocytic leukemia: clinical implications. BMC Med 2013;11:124. 
  6. Martínez-Trillos A, Quesada V, Villamor N, Puente XS, López-Otín C, Campo E. Recurrent Gene Mutations in CLL. Adv Exp Med Biol 2013;792: 87-107.
  7. Puente XS, López-Otín C. The evolutionary biography of chronic lymphocytic leukemia. Nat Genet 2013;45(3):229-31. 
  8. Campo E. Whole genome profiling and other high throughput technologies in lymphoid neoplasms—current contributions and future hopes [Revision]. Modern Pathology 2013;26:S97-S110. 
  9. Martín-Subero JI, López-Otín C, Campo E. Genetic and epigenetic basis of chronic lymphocytic leukemia. Curr Opin Hematol 2013;20(4):362-8.
  10. Ramsay AJ, Martínez-Trillos A, Jares P, Rodríguez D, Kwarciak A, Quesada V. Next-generation sequencing reveals the secrets of the chronic lymphocytic leukemia genome. Clin Transl Oncol 2013;15(1):3-8. 
  11. López C, Delgado J, Costa D, et al. NOTCH1 Mutations in Chronic Lymphocytic Leukemia with Trisomy 12. [Letter to Editor]. Genes Chromosomes Cancer 2012;51:1064-5.
  12. Quesada V, Ramsay AJ, Lopez-Otin C. Chronic lymphocytic leukemia with SF3B1 mutation. [Letter to Editor]. N Engl J Med 2012;366(26):2530.
  13. Campo E. New pathogenic mechanisms in Burkitt lymphoma. Nat Genet 2012;44(12):1288-9.

Collaborations

  1. Queirós AC, Villamor N, Clot G, Martinez-Trillos A, Kulis M, Navarro A, Penas EM, Jayne S, Majid A, Richter J, Bergmann AK, Kolarova J, Royo C, Russiñol N, Castellano G, Pinyol M, Bea S, Salaverria I, López-Guerra M, Colomer D, Aymerich M, Rozman M, Delgado J, Giné E, González-Díaz M, Puente XS, Siebert R, Dyer MJ, López-Otín C, Rozman C, Campo E, López-Guillermo A, Martín-Subero JI. A B-cell epigenetic signature defines three biologic subgroups of chronic lymphocytic leukemia with clinical impact. Leukemia 2015; 29(3):598-605
  2. Alioto TS, Buchhalter I, Derdak S, Hutter B, Eldridge MD, Hovig E, Heisler LE, Beck TA, Simpson JT, Tonon L, Sertier AS, Patch AM, Jäger N, Ginsbach P, Drews R, Paramasivam N, Kabbe R, Chotewutmontri S, Diessl N, Previti C, Schmidt S, Brors B, Feuerbach L, Heinold M, Gröbner S, Korshunov A, Tarpey PS, Butler AP, Hinton J, Jones D, Menzies A, Raine K, Shepherd R, Stebbings L, Teague JW, Ribeca P, Giner FC, Beltran S, Raineri E, Dabad M, Heath SC, Gut M, Denroche RE, Harding NJ, Yamaguchi TN, Fujimoto A, Nakagawa H, Quesada V, Valdés-Mas R, Nakken S, Vodák D, Bower L, Lynch AG, Anderson CL, Waddell N, Pearson JV, Grimmond SM, Peto M, Spellman P, He M, Kandoth C, Lee S, Zhang J, Létourneau L, Ma S, Seth S, Torrents D, Xi L, Wheeler DA, López-Otín C, Campo E, Campbell PJ, Boutros PC, Puente XS, Gerhard DS, Pfister SM, McPherson JD, Hudson TJ, Schlesner M, Lichter P, Eils R, Jones DT, Gut IG. A comprehensive assessment of somatic mutation detection in cancer using whole-genome sequencing. Nat Commun 2015;6:10001.
  3. Baliakas P, Hadzidimitriou A, Sutton LA, Rossi D, Minga E, Villamor N, Larrayoz M, Kminkova J, Agathangelidis A, Davis Z, Tausch E, Stalika E, Kantorova B, Mansouri L, Scarfò L, Cortese D, Navrkalova V, Rose-Zerilli MJ, Smedby KE, Juliusson G, Anagnostopoulos A, Makris AM, Navarro A, Delgado J, Oscier D, Belessi C, Stilgenbauer S, Ghia P, Pospisilova S, Gaidano G, Campo E, Strefford JC, Stamatopoulos K, Rosenquist R. Recurrent mutations refine prognosis in chronic lymphocytic leukemia. Leukemia 2015;29:329-36.
  4. Chalmers D, Nicol D, Nicolás P, Zeps N. A role for research ethics committees in exchanges of human biospecimens through material transfer agreementsJ Bioeth Inq 2014;11(3):301-6.
  5. Milius D, Dove ES, Chalmers D, Dyke SO, Kato K, Nicolás P, Ouellette BF, Ozenberger B, Rodriguez LL, Zeps N, Joly Y. The International Cancer Genome Consortium's evolving data-protection policiesNat Biotechnol 2014;32(6):519-23.
  6. Robles-Espinoza CD, Harland M, Ramsay AJ, Aoude LG, Quesada V, Ding Z, Pooley KA, Pritchard AL, Tiffen JC, Petljak M, Palmer JM, Symmons J, Johansson P, Stark MS, Gartside MG, Snowden H, Montgomery GW, Martin NG, Liu JZ, Choi J, Makowski M, Brown KM, Dunning AM, Keane TM, López-Otín C, Gruis NA, Hayward NK, Bishop DT, Newton-Bishop JA, Adams DJ. POT1 loss-of-function variants predispose to familial melanomaNat Genet 2014;46(5):478-81. 
  7. Berndt SI, Skibola CF, Joseph V, et al. (Benavente Y, Carracedo A, Lopez-Otin C, Bea S, Salaverria I, Martin D, Campo E, de Sanjose S). Genome-wide association study identifies multiple risk loci for chronic lymphocytic leukemia. Nat Genet 2013;45(8):868-76. 
  8. Alexandrov LB, Nik-Zainal S, Wedge DC, Aparicio SA, Behjati S, Biankin AV, Bignell GR, Bolli N, Borg A, Børresen-Dale AL, Boyault S, Burkhardt B, Butler AP, Caldas C, Davies HR, Desmedt C, Eils R, Eyfjörd JE, Foekens JA, Greaves M, Hosoda F, Hutter B, Ilicic T, Imbeaud S, Imielinsk M, Jäger N, Jones DT, Jones D, Knappskog S, Kool M, Lakhani SR, López-Otín C, Martin S, Munshi NC, Nakamura H, Northcott PA, Pajic M, Papaemmanuil E, Paradiso A, Pearson JV, Puente XS, Raine K, Ramakrishna M, Richardson AL, Richter J, Rosenstiel P, Schlesner M, Schumacher TN, Span PN, Teague JW, Totoki Y, Tutt AN, Valdés-Mas R, van Buuren MM, van 't Veer L, Vincent-Salomon A, Waddell N, Yates LR; Australian Pancreatic Cancer Genome Initiative; ICGC Breast Cancer Consortium; ICGC MMML-Seq Consortium; ICGC PedBrain, Zucman-Rossi J, Futreal PA, McDermott U, Lichter P, Meyerson M, Grimmond SM, Siebert R, Campo E, Shibata T, Pfister SM, Campbell PJ, Stratton MR. Signatures of mutational processes in human cancer. Nature 2013;500(7463):415-21. 
  9. Robles C, Poloczek A, Casabonne D, Gonzalez-Barca E, Bosch R, Benavente Y, Viscidi RP, de Sanjosé S. Antibody response to Merkel cell polyomavirus associated with incident lymphoma in the Epilymph case-control study in Spain. Cancer Epidemiol Biomarkers Prev 2012;21(9):1592-8. 
  10. International Cancer Genome Consortium, International network of cancer genome projects. Nature 2010;464(7291):993-8.